IDENTIFIKASI BAKTERI PADA PRODUK DARAH THROMBOCYTE CONCENTRATE

Widia  Rahmatullah; Ana  Dewi Lukita Sari; Rudina  Azimata Rosyidah; Roselina  Kuswandari; Arifin  Muflih; Reska  Handayani

doi:10.35328/kesmas.v13i1.2681

Authors

Widia Rahmatullah Politeknik Kesehatan Bhakti Setya Indonesia, Yogyakarta, Indonesia
Ana Dewi Lukita Sari Politeknik Kesehatan Bhakti Setya Indonesia, Yogyakarta, Indonesia
Rudina Azimata Rosyidah Politeknik Kesehatan Bhakti Setya Indonesia, Yogyakarta, Indonesia
Roselina Kuswandari Politeknik Kesehatan Bhakti Setya Indonesia, Yogyakarta, Indonesia
Arifin Muflih Politeknik Kesehatan Bhakti Setya Indonesia, Yogyakarta, Indonesia
Reska Handayani Universitas Negeri Padang, Padang, Indonesia

DOI:

https://doi.org/10.35328/kesmas.v13i1.2681

Keywords:

Thrombocyte Concentrate, Bacterial contamination, NA Media, MCA Media

Abstract

TThe Thrombocte Concentrate component is the blood component that is most easily contaminated with bacteria due to its processing and storage at room temperature. Blood or blood products that are ready to be transfused must be safe and free from the risk of infection by infectious diseases and contamination by microorganisms. Sources of bacterial contamination can come from donor skin that is less than aseptic, donor bacteremia and processing of blood products. This type of research is Pre-Experiment. The design of this research is One-Shot Case Study. In One-Shot Case Study design. The sample in this study used 1 Thrombocyte Concentrate blood product. Then it was replicated into 10 samples for Nutrient Agar media and 10 samples for Mac conkey Agar media. From the results of research conducted on 10 samples of Nutrient Agar (NA) media, it was found that there was bacterial growth in all petri dishes on Nutrient Agar (NA) media. ), while in petri dish number 10 it was found that 10% of gram-positive bacteria were in the form of bacilli (rods). From the results of research conducted on 10 samples of Mac Conkey Agar (MCA) media, no bacterial growth was found in all of the MCA media petri dishes. There was contamination of Gram-Positive bacteria in the form of Cocci (Rounds) and Bacillus (Sticks) in Thrombocyte Concentrate blood products using NA media in 10 samples out of the total samples examined were 10 samples of NA media. There was no Gram-Negative bacterial contamination in Thrombocyte Concentrate blood products using MCA media in 10 samples from the 10 samples examined.

References

Achmad Tjiptoprajitno, N., & Ketut Sudiana, I. (2012). Analisis Produk Darah Thrombocyte Concentrate di Palang Merah Indonesia Surabaya (Analysis of Blood Product Trombocyte Concentrate in Red Cross Blood Donor Unit Surabaya). JBP, 14(3), 145–152.

Agzie, M., Niguse, S., Tsegay, E., Kahsay, G., & Mahmud, M. A. (2019). Bacterial contaminants of stored blood and blood components ready for transfusion at blood banks in Mekelle, Northern Ethiopia. BMC Research Notes, 12(1), 1–6. https://doi.org/10.1186/s13104-019-4217-0

Astuti, D., & Ayu Maharani. (2014). Identifikasi Bakteri Yang Mengontaminasi Konsentrat Trombosit. Jurnal Ilmu Dan Teknologi Kesehatan, 2(1), 61–67.

Btari Christiyani Kusumaningrum, S., & Sepvianti, W. (2020). Identifikasi Bakteri Kontaminan Pada Produk Darah Thrombocyte Concentrate. Syifa’ MEDIKA, 10(2), 117–123.

Destik Wulandari, D. P. (2019). Identifikasi dan Dan Karakterisasi Bakteri Amilolitik Pada Umbi Colocasia Esculenta L. Secara Morfologi, Biokimia, Dan Molekuler. Jurnal Bioteknologi Dan Biosains Indonesia, 6(2), 247–258. http://ejurnal.bppt.go.id/index.php/JBBI

Farzad, B. B. , F. B. , Z. B. , N. A. , & M. K. B. (2016). Bacterial contamination of platelet products in the Blood Transfusion Center of Isfahan. Journal GMS Hygiene and Infection Control, 11(1), 1–4.

Haass, K. A., Sapiano, M. R. P., Savinkina, A., Kuehnert, M. J., & Basavaraju, S. V. (2019). Transfusion-Transmitted Infections Reported to the National Healthcare Safety Network Hemovigilance Module. Transfusion Medicine Reviews, 33(2), 84–91. https://doi.org/10.1016/j.tmrv.2019.01.001

Haney, E. F., Trimble, M. J., Cheng, J. T., Vallé, Q., & Hancock, R. E. W. (2018). Critical assessment of methods to quantify biofilm growth and evaluate antibiofilm activity of host defence peptides. Biomolecules, 8(2), 1–22. https://doi.org/10.3390/biom8020029

He, Z., Che, J., Wang, Q., Chen, S., Chen, Q., Yu, L., Zou, J., & Hu, Y. (2018). Bacterial contamination of platelet products in Dongguan Blood Center, Guangdong Province of China. Annals of Blood, 3(36), 1–5. https://doi.org/10.21037/aob.2018.08.01

Horth, R. Z., Jones, J. M., Kim, J. J., Lopansri, B. K., Ilstrup, S. J., Fridey, ; Joy, Walter, ;, Kelley, E., Stramer, S. L., Nambiar, A., Lynn Ramirez-Avila, ;, Nichols, A., Garcia, W., Kelly, ;, Oakeson, F., Vlachos, N., Mcallister, G., Hunter, R., Nakashima, A. K., … Basavaraju, V. (2017). Morbidity and Mortality Weekly Report Fatal Sepsis Associated with Bacterial Contamination of Platelets-Utah and California, August 2017. MMWR. Morbidity and Mortality Weekly Report, 67(25), 718–722, 67(25), 718–722. https://stacks.cdc.gov/view/cdc/56097

Jawetz E, M. J. A. E. (2008). Mikrobiologi Kedokteran Edisi 23 (23rd ed.). Salemba Medika.

Kemenkes RI. (2015). Peraturan Menteri Kesehatan Republik Indonesia No 91.

Putri MH, Yodong, & Sukini. (2017). Bahan Ajar Keperawatan Gigi Mikrobiologi.

Putri Welkriana, W., & Dira Irnameria. (2019). Hitung Angka Kuman Darah Pada Bank Darah Di Rumah Sakit Dr. M Yunus Bengkulu. Avicenna: Jurnal Ilmiah, 56(1), 1–59.

Sugiyono. (2015). Metode Penelitian Kuantitatif, Kualitatif, dan R&D. ALFABETA,

Thyer, J., Perkowska‐Guse, Z., Ismay, S. L., Keller, A. J., Chan, H. T., Dennington, P. M., Bell, B., Kotsiou, G., & Pink, J. M. (2018). Bacterial testing of platelets – has it prevented transfusion‐transmitted bacterial infections in Australia? Vox Sanguinis, 113(1), 13–20. https://doi.org/10.1111/vox.12561

Triana, S., & Zaymi, S. (2021). Platelet Counts on the 1 st and 5 th Days in Thrombocyte Concentrate (TC) Products. Advances in Health Sciences Research, , 36, 306–308