POTENSI EKSTRAK BUNGA PISANG KEPOK (MUSA PARADISIACA L.) SEBAGAI ANTIOKSIDAN DENGAN METODE DPPH (1,1-DIFENIL-2-PIKRILHIDRAZIL)

Farisya Nurhaeni; Amelia Handayani Burhan; Fydika Nurul Riani Sumarjo; Aditya Fitriasari

doi:10.35328/kesmas.v14i1.2917

Authors

Farisya Nurhaeni Politeknik Kesehatan Bhakti Setya Indonesia, Yogyakarta, Indonesia
Amelia Handayani Burhan Politeknik Kesehatan Bhakti Setya Indonesia, Yogyakarta, Indonesia
Fydika Nurul Riani Sumarjo Politeknik Kesehatan Bhakti Setya Indonesia, Yogyakarta, Indonesia
Aditya Fitriasari Politeknik Kesehatan Bhakti Setya Indonesia, Yogyakarta, Indonesia

DOI:

https://doi.org/10.35328/kesmas.v14i1.2917

Keywords:

antioxidant, kapok banana flower, DPPH, chloroform

Abstract

Kepok banana flower has potential as an antioxidant so it can inhibit free radical activity. Previous research stated that banana flower extract (Musa acuminate) has an IC₅₀value of 13.21 µg/ml, which means it has very strong antioxidant activity. Therefore, a study was carried out to fractionate the ethanol extract of kepok banana flowers and determine the antioxidant activity of each of these fractions. It is hoped that with this research a purer fraction will be obtained to increase the antioxidant activity. Extraction was carried out by maceration using 96% ethanol, while fractionation was carried out by chloroform. Testing the antioxidant activity using the DPPH method with UV-VIS spectrophotometry. The wavelength used is 517 nm. Extraction was carried out on 30 grams of kepok banana flower powder with 96% ethanol solvent so that 1.27 grams of the thick extract was obtained. Fractionation was carried out on 500 mg of ethanol extract from kepok banana flowers which produced 0.21 gram of soluble fraction and 0.28 gram of chloroform insoluble fraction. The results showed that the IC50 values of ethanol extract, soluble fraction and chloroform insoluble fraction of kepok banana flower extract were 26.00 µg/ml, 236.17 µg/ml, and 18.70 µg/ml respectively. It can be concluded that the ethanol extract and chloroform insoluble fraction of kepok banana flowers have very strong antioxidant activity, while the chloroform soluble fraction has weak antioxidant activity.

References

Andriani, D., & Murtisiwi, L. (2020). Uji Aktivitas Antioksidan Ekstrak Etanol 70 % Bunga Telang ( Clitoria ternatea L ) dari Daerah Sleman dengan Metode DPPH Antioxidant Activity Test of 70 % Ethanol Extract of Telang Flower ( Clitoria ternatea L ) from Sleman Area with DPPH Method. PHARMACON: Jurnal Farmasi Indonesia, 17(1), 70–76.

Dewatisari, W. F. (2020). Perbandingan Pelarut Kloroform dan Etanol terhadap Rendeman Ekstrak Daun Lidah Mertua (Sansevieria trifasciata prain.) Menggunakan Metode Maserasi. Prosiding Seminar Nasional Biologi Di Era Pandemi COVID-19, September, 128–132.

Eko Murwanto, P., & Santosa, D. (2012). Uji Aktivitas Antioksidan Tumbuhan Cynara scolimus L., Artemisia china L., Borreria repensDC., Polygala paniculata L. Hasil Koleksi dari Taman Nasional Gunung Merapi dengan Metode Penangkapan radikal DPPH (2,2-Difenil-1-Pikrilhidrazil). Majalah Obat Tradisional, 17(3), 53. https://jurnal.ugm.ac.id/TradMedJ/article/view/8011/6220

Ferdinan, A., & Prasetya, A. B. (2018). Uji Aktivitas Antioksidan dari Ekstrak Jantung Pisang Kepok (Musa paradisiaca L.) Pontianak. Jurnal Ilmiah Ibnu Sina, 3(1), 88–96.

Jami’ah, S. R., Ifaya, M., Pusmarani, J., & Nurhikma, E. (2018). Uji Aktivitas Antioksidan Ekstrak Metanol Kulit Pisang Raja (Musa Paradisiaca sapientum) Dengan Metode DPPH (2,2-Difenil-1-Pikrilhidrazil). Jurnal Mandala Pharmacon Indonesia, 4(1), 33–38. https://doi.org/10.35311/jmpi.v4i1.22

L. Prior, R., Wu, X., & Schaich, K. (2005). Standardized Methods for the Determination of Antioxidant Capacity and Phenolics in Foods and Dietary Supplements. Journal of Agricultural and Food Chemistry, 53(10), 4290–4302. https://doi.org/10.1021/jf0502698

Nurhaeni, F., Yuliana, P., & Fitriasari, A. (2019). Skrining Fitokimia dan Uji Aktivitas Antioksidan Pelepah Batang dan Bunga Pisang Kepok (Musa acuminatae, L.). Jurnal Ilmu Kesehatan Bhakti Setya Medika, 4(1), 29–35.

Rahmi, A., Hardi, N., & Hevira, L. (2022). Aktivitas Antioksidan Ekstrak Kulit Pisang Kepok, Pisang Mas Dan Pisang Nangka Menggunakan Metode DPPH. Jurnal Ilmu Farmasi Dan Farmasi Klinik (JIFFK), 18(2), 77–84. https://doi.org/10.31942/jiffk.v18i2.5961

Rampe, M. J., & Tombuku, J. L. (2015). Pengujian fitokimia dan toksisitas ekstrak etanol jantung pisang kepok (Musa paradisiaca LINN .) dengan metode Brine Shrimp Lethality Test (BSLT). Jurnal Sainsmat, 4(2), 136–147.

Rinaningsih. (2012). Optimasi Pelarut Pengembang dalam Pemisahan Benzil Asetat dari Ekstrak Bunga Tanaman Melati. Sains Dan Matematika, 1(1), 20–23. https://journal.unesa.ac.id/index.php/sainsmatematika/article/view/23

Sari, M., Ulfa, R. N., Marpaung, M. P., & Purnama. (2021). Penentuan Aktivitas Antioksidan dan Kandungan Flavonoid Total Ekstrak Daun Papasan (Coccinia grandis L.) Berdasarkan Perbedaan Pelarut Polar. KOVALEN: Jurnal Riset Kimia, 7(1), 30–41. https://doi.org/10.22487/kovalen.2021.v7.i1.15437

Sugiyono. (2019). Metode Penelitian dan Pengembangan (Research and development/R&D) (4th ed.). Alfabeta.

Yuslianti, E. R. (2018). Pengantar Radikal Bebas dan Antioksidan (1st ed.). Deep Publish.

Yuswi, N. C. R. (2017). Ekstraksi Antioksidan Bawang Dayak (Eleutherine Palmifolia) Dengan Metode Ultrasonic Bath (Kajian Jenis Pelarut Dan Lama Ekstraksi). Jurnal Pangan Dan Agroindustri, 5(1), 71–79. https://jpa.ub.ac.id/index.php/jpa/article/view/499